Elsevier

Molecular Metabolism

Volume 6, Issue 4, April 2017, Pages 317-326
Molecular Metabolism

Original article
Weight loss independent changes in adipose tissue macrophage and T cell populations after sleeve gastrectomy in mice

https://doi.org/10.1016/j.molmet.2017.02.004Get rights and content
Under a Creative Commons license
open access

Highlights

  • Sleeve gastrectomy in mice altered the adipose leukocyte populations.

  • CD11c macrophages were selectively upregulated independent from weight loss.

  • All identified T cell populations increased manifold independent from weight loss.

  • The dendritic cell fraction was decreased below lean control levels with surgery.

Abstract

Objective

In addition to adipocytes, adipose tissue contains large numbers of immune cells. A wide range of evidence links the activity of these cells to regulation of adipocyte and systemic metabolic function. Bariatric surgery improves several aspects of metabolic derangements and at least some of these effects occur in a weight-loss independent manner. We sought to investigate the impact of vertical sleeve gastrectomy (VSG) on adipose immune cell frequencies.

Methods

We analyzed the frequencies of immune cells within distinct adipose tissue depots in obese mice that had VSG or sham surgery with a portion of the latter group pair-fed such that their body mass was matched to the VSG animals.

Results

We demonstrate that VSG induced a shift in the epididymal adipose tissue leukocyte profile including increased frequencies of CD11c macrophages, increased frequencies of T cells (CD4+, CD8+, and CD4/CD8 T cells all increased), but a significantly decreased frequency of adipose tissue dendritic cells (ATDC) that, despite the continued high fat feeding of the VSG group, dropped below control diet levels.

Conclusions

These results indicate that VSG induces substantial changes in the immune populations residing in the adipose depots independent of weight loss.

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Keywords

Sleeve gastrectomy
Adipose tissue
Macrophages
T cells
Dendritic cells

Abbreviations

ATDC
adipose tissue dendritic cell
ATM
adipose tissue macrophage
eWAT
epididymal white adipose tissue
FFA
free fatty acids
HFS
high fat sham
iWAT
inguinal white adipose tissue
SVC
stromal vascular cells
VSG
vertical sleeve gastrectomy

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