Original ArticleNNT reverse mode of operation mediates glucose control of mitochondrial NADPH and glutathione redox state in mouse pancreatic β-cells
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Keywords
C57BL/6J mice
C57BL/6N mice
Glucose metabolism
GRX1-roGFP2
Insulin secretion
Mitochondrial shuttles
Pancreatic islet
Redox-sensitive GFP
Stimulus-secretion coupling
Abbreviations
AT2
aldrithiol
CMV
cytomegalovirus
Dz
diazoxide
DTT
dithiotreitol
FCCP
carbonyl cyanide-p-trifluoromethoxyphenylhydrazone
GSIS
glucose stimulation of insulin secretion
GRX1
glutaredoxin 1
[Ca2+]i
intracellular Ca2+ concentration
IDH
isocitrate dehydrogenase
KRB
Krebs solution
ME
malic enzyme
WT
wild-type
NNT
nicotinamide nucleotide transhydrogenase
OCR
oxygen consumption rate
Cited by (0)
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Laila R.B. Santos and Carole Muller contributed equally to this work.
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Present address: Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany.
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Present address: MitoCare Center, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA.
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Present address: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
© 2017 The Authors. Published by Elsevier GmbH.