Elsevier

Molecular Metabolism

Volume 6, Issue 7, July 2017, Pages 651-663
Molecular Metabolism

Original Article
Mitochondrial transcription factor B2 is essential for mitochondrial and cellular function in pancreatic β-cells

https://doi.org/10.1016/j.molmet.2017.05.005Get rights and content
Under a Creative Commons license
open access

Highlights

  • Loss of TFB2M leads to mitochondrial dysfunction and impaired insulin secretion.

  • There was an effect of gene dosage on Tfb2m expression and function.

  • TFB2M plays a key role in cellular and mitochondrial function in pancreatic β-cells.

Abstract

Objective

Insulin release from pancreatic β-cells is controlled by plasma glucose levels via mitochondrial fuel metabolism. Therefore, insulin secretion is critically dependent on mitochondrial DNA (mtDNA) and the genes it encodes. Mitochondrial transcription factor B2 (TFB2M) controls transcription of mitochondrial-encoded genes. However, its precise role in mitochondrial metabolism in pancreatic β-cells and, consequently, in insulin secretion remains unknown.

Methods

To elucidate the role of TFB2M in mitochondrial function and insulin secretion in vitro and in vivo, mice with a β-cell specific homozygous or heterozygous knockout of Tfb2m and rat clonal insulin-producing cells in which the gene was silenced were examined with an array of metabolic and functional assays.

Results

There was an effect of gene dosage on Tfb2m expression and function. Loss of Tfb2m led to diabetes due to disrupted transcription of mitochondrial DNA (mtDNA) and reduced mtDNA content. The ensuing mitochondrial dysfunction activated compensatory mechanisms aiming to limit cellular dysfunction and damage of β-cells. These processes included the mitochondrial unfolded protein response, mitophagy, and autophagy. Ultimately, however, these cell-protective systems were overridden, leading to mitochondrial dysfunction and activation of mitochondrial-dependent apoptotic pathways. In this way, β-cell function and mass were reduced. Together, these perturbations resulted in impaired insulin secretion, progressive hyperglycemia, and, ultimately, development of diabetes.

Conclusions

Loss of Tfb2m in pancreatic β-cells results in progressive mitochondrial dysfunction. Consequently, insulin secretion in response to metabolic stimuli is impaired and β-cell mass reduced. Our findings indicate that TFB2M plays an important functional role in pancreatic β-cells. Perturbations of its actions may lead to loss of functional β-cell mass, a hallmark of T2D.

Keywords

Mitochondrial metabolism
Pancreatic β-cells
Insulin secretion

Cited by (0)

5

Lisa M. Nicholas and Bérengère Valtat contributed equally to this work.